Monday 06/11/12

10:00 A – 7:00P, 8:30P-11:30P

• Hao lab meeting
• in situs day 1 (by KE): protein structure between hox targets
• Fixing AOTF problem with STORM2.  Between beam shape and AOTF, no usable light is passed.
• AOTF removed from table.
• analyze probe test results.
• PausePlayPlayPrev|Next

  

  tou_bio

  

  5E-bio_AbdB3-dig

  

  7E-bio_8E-dig

  

  8E-bio_7E-dig

  

  AbdA-bio

  

  AbdB1-bio_Fab8-dig

  

  AbdB3-bio_5E-dig

  

  Dfd2_AbdB2

  

  Ubx-bio

  

  Ubx-dig

  

  AbdA-dig

  

  Antp-dig

  

  en-dig

  

  Epc-dig

  

  Epc_bio

  

  AbdB1-digR_Fab8-bioG

  

  en-bio

  

  inv-bio

  

  taf-digR_tub-bioG

  

  taf-bio_tub-dig

• Singer protocol (Nature Protocols 2012) suggests EtOH storage enhances mRNA stains by increasing penetration through membranes.  This may explain the difference between some of my test stains on stored embryos and difficulty detecting in fresh embryos.  Maybe longer FISH incubation will help.
• STORM more microtubules and beads
• .ini files written by modify ini are larger but have identical text?  must be a whitespace problem.