10:00 A-5:00P, 9:30P – 12:00A
- Flip collection cages, 10:30A
- still working on z-calibration bug. updated write_minimal_dax_ini file seems to be the only script that won’t run correctly. Putting max_displacement = 1 causes errors in otherwise functional script. May need to run z-cal with max_displacement = .0005 and change this to = 1 before running 3D beads and data.
- was missing num2str() conversion for pars in max_displacement in modified write…ini.m script.
- writing script to convert text.
- in situs day 2 with KE: staining hox pairs:
- [spreadsheet 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE 602 302 sheet=5]
- Confocal test new antibodies: R&D Systems dk a-m and dk a-rb look very clean. Jackson anti-ch from Colenso lacks foci but has higher nuclear background.
- Confocal test PCNA antibodies. abcam gives foci, Santa Cruz gives global nuclear