Tuesday 06/12/12

10:00 A-5:00P, 9:30P – 12:00A

  • Flip collection cages, 10:30A
  • still working on z-calibration bug.  updated write_minimal_dax_ini file seems to be the only script that won’t run correctly.  Putting max_displacement = 1 causes errors in otherwise functional script.  May need to run z-cal with max_displacement = .0005 and change this to = 1 before running 3D beads and data.
  • was missing num2str() conversion for pars in max_displacement in modified write…ini.m script.
  • writing script to convert text.
  • in situs day 2 with KE: staining hox pairs:
  • [spreadsheet 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE 602 302 sheet=5]
  • Confocal test new antibodies: R&D Systems dk a-m and dk a-rb look very clean.  Jackson anti-ch from Colenso lacks foci but has higher nuclear background.
  • Confocal test PCNA antibodies.  abcam gives foci, Santa Cruz gives global nuclear
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