10:10 A – 7:30P, 8:20P – 10:45P
- Flip collection cages.
- Meeting with Ting and Xiaowei.
- KE miniprep cg5… cg10… and bxd samples. lab colonies did not grow in liquid culture? Sent samples for sequencing.
- STORM of Pc6_cntrl
- Label new dk-rb-750 and dk-rt-488 antibodies
- Successful 3 position STORM run on Pc6. Second (intended O/N) STORM run starts overwriting 1st run. Restarted. Hal then freezes on movie 5. Restarted run again.
- Sequential imaging of snail-promoter BACs using Zeiss 700.
- Rewrite to Dave/HCBI about ELYRA 710 use.
- DNA FISH at 1:3 dilution with BAC probes:
- Staining centromere labeled embryos with Dm01 and H3K27me3 (see if this works after GA fixation. Not sure status of separated embryos).
- KE fix embryos (2-8 hr).
- Reduced confocal scan step size to from .25, to .2 to .15 to correct for lens expansion / z-drift errors. (trouble with sequential imaging).