Thursday 08/02/12

Posted on August 2, 2012 by admin

9:50A — 6:20, 8:20p – 11:55P

  • Confocal O/N — not enough sections / .17 spacing too close to make it through nuclear layer.  trashed image.
  • STORM O/N — Hal froze on movie 48.  Sent crash report to Hazen.  Shut down microscope.
  • Flip fly cages, 10:00A
  • Sequencing results:  bxd-1 (on minus, seq +/+ with M13R)
  • -bxd-2: CG5220 +, +/+  (all with M13R)

    -CG5220-1: CG5220, +, +/+

    -CG5220-2: CG5220, +, +/+

    -CG10326-1: CG10326, +, +/-

    -CG10326-2: CG10326, +, +/-

  • DNA FISH day 2. En locus DNA FISH fails again.
  • antibody labeling  ‘day 3’ for centromere FISH (KE?)
  • KE in situs day 1
  • [spreadsheet 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE 602 402 sheet=8]
  • previous cut samples of 6/24 stains of slide 5 look good by Turnkey.  Etched for STORM.
  • Confocal imaging of m197 with Zeiss700.
  • STORM4 AOTF not responding :(.
  • KE Fix embryos 6P (1-8 hrs).
  • STORM sample 5 from 6/24.  17 min etch not sufficient — substantial patches visible in 647 channel.  Try 22 min?
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