Saturday 11/03/12

10:45 A –

Writing

• Write BJ review
  • Drosophila PWMs TF binding motifs FlyFactor
  • Ecoli TF binding + PWMs here (might be something better)
• Finish first draft through of enhancer integration section.  On to promoters next.

DFISH on cell culture

• remove probes and re-freeze for reuse
• test on Turnkey staining.  Looks good in well 1 and 7.  Unless the probe has evaporated from well 8 and recondensed in the other wells, the DNA FISH in cells appears to be working.  Pretty easy protocol, shoud repeat without the positive control just for test (or add leave a no-probe negative control again).
• post-hybe washes

Fly work

• Flip collection cages, 6:15 PM (sim, MTD, and 3 cages MTD x SCM).
• No virgin collection.

 Embryo staining