9:30A -10:00P
Overview of today
- Flip sim[D] plate (9:30A)
- Colenso Lab meeting
- Age sim[D] plate and 3 MTD x SCM plates
- Fix sim[D] and SCM embryos. Should be ready for in situs
- Working on Francis lab group meeting presentation / research planning
- Bustamante seminar
cell culture DFISH methods development
- Attempting staining on coverslips. Test sample works with WGA-488 + hoechst — cells still attached densely to coverslip after washing coverslip, adhereing coverslip to glass slide (for ~10 min + imaging) and detaching from glass slide for post-wash. Coverglass mounts nicely in prolong
- Should use 18mm coverglass in 12-well plates. 22mm fits perfectly and is perfectly impossible to remove coverglass without inverting (loosing solution), or by chipping/ breaking coverglass to create handle (current solution).