9:30 A -8:00P, 9:30-11:00P
- check STORM. — run finished without losing focus.
- Summary of inv run: Unfortunately 514 doesn’t work either as a double label. inv-Epc male a lot of promising double spots, but it’s a litter harder to sort out what’s what. We’ll have to see in the analysis. These double labels look so good in confocal, it’s a real shame they get lost so fast for STORM. Maybe IR800 will work better….
- Attempt STORM of DFISH H3, H3K27, H3K27ac. Sample too degraded in 4 days (mounted Sunday night). Should have post-fixed and imaged sooner
- Fixed issue with mounting hard drives. See details in post.
- Shoot 2D beads for chromatic alignment
- downloading STORM data
Communication
- Review NSF proposal and send back to team.
- Need a ICAM member to submit an ICAM proposal. This might be a substantial problem.
- Complete go ahead from ML on snail proposal. Need discuss possability of including Mounia’s data or not.
- Levine sends back proposed figures. Need detailed description of model and ready to go.
- Got protocols from Brian. Ordered glycogen and Kaka taq.
- Call Prof. Bender. Schedule meeting tomorrow at 11A (Bender’s office)
- working on slides for meeting with Bender
Writing BJ review
- working on modeling for effect of binding site removal under different models of binding site interactions.
- add results figure.
- cooperative binding changes position of threshold and steepness of switching
- promoter synergy increases affects total output without affecting threshold/steepness much
- redundant sites have minor effect on location of threshold and don’t affect max output. They do make the system more robust to stochastic noise that leads to temporary variations in binding site occupancy.
Fly work
- Check fly crosses. Espl/sim x TM2/TM6 look good
- All Act5 crosses have larvae (though very different numbers). Except Pc1, which had only 1 male who doesn’t look happy (stuck to food at present).
- Cleared and moved Sp/CyOZ; TM2,Ubx/TM3,Tb to 18C for virgin collection.
- >99% recovery of Drosophila at 4C for > 12hrs
- # collect virgin TM2,Ubx/TM6,Tb