11:00 A – 8:30P,
9:30P-11:00P (remote)
Fly work
- collect virgins
- non-Sp CyO males of Esc not obviously extra sex combs (only 1 male so far). Need to check bristly number and frequency expected (see Vivek’s paper).
- new bottles of MTDs
- PM virgin collection. Good Esc male phenotypes.
stock vials dead:
- yw (replace from bottles)
- UAS-GAP43:mEOS (don’t need)
- yw ACT-GAL4 (don’t need anymore)
- sna[18]/CyO (restock from DB attempt)
Coding
- integrate chromatic warp into STORMfinderBeta
- Not fully integrated yet: 11P.
STORM
- New samples: Bx-C-cy5, Pc-488 round 2. Fab-7-cy5, Pc-750 round 2.
- Issues with back-optics alignment: be sure pinhole at end of beam expansion tube is tight, to give a good diffraction pattern for aligning beam on camera. Otherwise the spot position on the camera is not very sensitive to mirror locations.
- Re-square fields for Qaudview (substantially off center)
- Fully warmed slides drift WAY less (duh). Be sure to warm bead slides to RT.
- O/N imaging of Bx-C-cy5, Pc-488 round 2.