results
prior to extraction
- y prot contr = too high
- BP 1/2 PCR = 38 ug/mL 7:20 P, reusing standards, 10.9
- BP control = 72.4 ug/mL
- BP = 35 ug/mL
- Y-T em 1/2 = 9.16 ug/mL. 7:20P, reusing standards, = 15.3.
- YP control 67.3 ug/mL in 250 uL
- yp = 73.7 ug/mL
extracted Qubit [Nanodrop]
- (Only loaded 5 uL, instead of 20 for some samples, corrected with a 4x multiplier indicated)
- Y-T em 1/2 = 37 ug/mL [143.6 ng/uL]
- BP = 37 ug/ mL [208 ng/uL]
- YP = 26.8 (*4) ~ 100 ug/uL [320 ng/uL]
- YP cont = 27.2. (*4) = 97.0 ug/mL in 150 uL [425 ng/uL]
- BP cont = 16.8 ug/mL (*4) = 64 ug/mL [316 ng/uL]
- BP pcr 1/2= 28.0 (*4) = 112 ug/mL [374 ng/uL]
- BA = 51.0 ug/mL [576 ng/uL]
- YA = 22.8 ug/mL [579 ng/uL]
digested
- y 1mL filtered = 147.3 ng/uL
- y 1mL pre-filter = 286.0 ng/uL, very ugly curve though
- y 10mL filtered = 226.4 ng/uL,
- y 10 mL pre-filtered = 20g/ng/uL, even uglier curve, peak at 230 not 260
- B 10mL filtered = 244.6 ng/uL
- B 10 mL pre-filtered = 525.9 ng/uL ugly curve
T7 try 2 (qubit) [Nanodrop of qubit sample]
- PPT-Control T=0, 7.72 ng/uL
- PPT T=0, 6.09 ng/uL
- PPT-Control T=1hr, 2.33 ng/uL
- PPT T =0, 2.78 ng/uL
- y sample nanodrop: 91.6
- ycontrol = 112 ng/uL
Notes from yesterday
yppt TOO HIGH >600 ng/uL YT 318 ng/mL => 21.2 ug/mL Y NTP 351 ng/mL => 23.4 ug/mL Y 2xP 38 ug/mL
B asy 37 ug/mL B ppt cntrl = 60.5 ug/mL y ppt cntrl = 54.4 ug/mL y ppt prot = 56.8 ug/mL B ppt prot = 58.8 ug/mL y asym = 57.1 ug/mL