Saturday 04/13/13

3:00 P – 8:00 P, 9:00 P – 11:00 P

Working on review

• Finalize discussion
• Touch up figures
• Touch up figure captions
• reread once through
• Send to Stas, Peter, Carl and David for comments

OligoPaint troubleshooting

• Qubit data
• 10% denaturing gel. Lanes:

Gel order

1. y 1ml digest
2. y 10ml digest
3. b 10ml digest
4. y assym
5. b assym
6. B-PPT T7
7. B-PPT control T7
8. y-T-em T7
9. Y-PPT ?
10. Y-PPT control

Summary, troubleshooting thoughts so far:

• All the recent PCR reactions have a large pool of DNA that won’t enter gels. Neither PAGE nor Agarose
• Filteration columns are quick and easy, but seem to lose almost all the DNA except the stuff that doesn’t enter gels.
• 50% loss in EtOH precipitation when no glycogen in initial precipitation. (Maybe it was the 50 mL eppendorf format).