denaturing diagnostic gels

Gel 1, 5% Agarose, 200 V in sodium-Borax (300 V melts gel).

Gel 2, 15% Urea, 120V 2 hrs

Gel 3 15% Urea gel, 120 V 50 min in 37C room

Gel 4, 10% precast Urea denaturing gel, 120 V, 45 min