experimental oligopaint PCR 3

Posted on April 14, 2013 by admin

Samples (1 mL scale)

  1. y nicked 2x Ya-primer
  2. b nicked 1x Ba-primer + 1 x CPPT
  3. ubx nicked 1x UBx-adapter-primer + 1x CPPT
  4. y PPT: 5 uL YA + 20 uL PPT
  5. y PPT control: 5 uL YA + 20 uL CPPT
  6. y assym: 10 uL YA + 10 uL CPPT (No YR)
  7. b PPT : 5 uL BA + 20 uL PPT
  8. b PPT control: 5 uL BA + 20 uL CPPT
  9. b assym: 10 uL BA + 10 uL PPT (no BR)
This entry was posted in probe and plasmid building. Bookmark the permalink.