Sunday 04/14/13

5:30 P – 8:00P

OligoPaint

• Analyzing previous gel images
• Conclusion: something went weird, possibly with original PCR used to seed most of the second round PCRs.
• Plan
  1. Repeat PCRs from scratch
  2. Run diagnostic denaturing gels at each step in addition to measuring concentrations. Have to know what species are there.
  3. Just put part of one sample on clean up column (after measuring concentration). Use gel compare to DNA ladder loaded on clean-up column.
• Setting up new PCRs (round3)
• Running diagnostic gel on Raw PCR product (5 uL PCR product + 5 uL loading buffer). Loaded 5 mL on 10% pre-cast Urea PAGE gel. running at 120 V.