Tuesday 04/16/13

9:30 A – 8:00 P, 9:00P – 12:00 A

OligoPaints

• Set up EtOH precipitation of samples 1-5. In -20C, ~10:00 A.
• Pour 5% agarose Na-Borax gel to try to diagnose issue with ladder: not all bands showing up on urea PAGE gel.
• Run gel
• Running gel at 240 V for another 30min. Gel melts. 🙁

Nanodrop

• L after filter = ~16 ng/uL (very ugly, ddH2O blank). 18 ng/uL (
• #4 filtered 25 ng/ul, very ugly
• #5 filtered 35 ng/uL very ugly, shoulder on a downward slope.

Evening gel lanes:

1. #1 EtOH recovered
2. #2 EtOH recovered
3. #3 EtOH recovered
4. #4 EtOH recovered
5. #5 EtOH recovered
6. ladder
7. #6
8. #7
9. #8
10. #9

Project 2

• See notes