Monday 04/15/13

Posted on April 15, 2013 by admin

9:30 A – 8:00 P, 9:00 P – 11:55 P

Meetings

  • group meeting (notes)
  • journal club (notes)
  • discussion with Xiaowei (notes)

Gels

  • At least 3 ways to screw up gels
  1. APS really needs to fresh, (try 140 uL instead of 38 uL)?
  2. mix up BME and TMED
  3. nicely cast gels don’t refirgerate — Urea percepitates and creates air bubbles.
    oligopaint_PCR3

* (gel lanes as numbered below, 1-10 left to right).

Nanodrop

  1. y nicked 2x Ya-primer [358 ng/ul]
  2. b nicked 1x Ba-primer + 1 x CPPT [363]
  3. ubx nicked 1x UBx-adapter-primer + 1x CPPT [360]
  4. y PPT: 5 uL YA + 20 uL PPT [356]
  5. y PPT control: 5 uL YA + 20 uL CPPT [354]
  6. y assym: 10 uL YA + 10 uL CPPT (No YR) [355]
  7. b PPT : 5 uL BA + 20 uL PPT [360]
  8. b PPT control: 5 uL BA + 20 uL CPPT [359]
  9. b assym: 10 uL BA + 10 uL PPT (no BR) [346]
  10. 10 mM dNTPs 5,330 ng/uL

Testing cleanup column

  • (#4) 150 uL of y PPT PCR prod + 750 uL binding buffer
  • (#5) 150 uL y CPPT PCR prod + 750 uL binding buffer
  • Doesn’t look like any DNA detectable on denaturing gel.

New project with Xiaowei

  • setting up coding with Steven
  • sent project schema outline to team
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