9:00 A – 10:33 P
OligoPaint Probe making
- Spin down crushed gels and aspirate fluid.
- Attempt to nanodrop. ~200/UV peak, readings probably meaningless. Could Qubit but hopefully these are single-stranded now and shouldn’t read.
nanodrop
- no 260 peak, so reading maybe useless, strong 200 peak.
- U short: 5
- U long: 11
- AB short*: 7 (has loading dye as well)
- AB long: 9
- O/N PCR of PPT and CPPT finished
- Pour gel
- Gel results:
To Do
Computer
- Consolidated Ph Data on to Alistair11.
- Cleared all data off of Alistair13, can go back to being mobile drive
- Moving en-emb to Alistair10 from Alistair12.
STORM analysis
- Insight batch analysis gives error
cannot save configuration file… but still seems to run. - Insight is not in fact analyzing correctly — list.bin file has only one molecule per frame instead of all the ones fit by the parameter choice.
- Insight parameter no-Z fit seems to lead to screwed up mlists output STORMfinderBeta
- Alistair9 spooling to Cajal has trouble running more than 4 version of Insight at once.
- ReadDaxBeta for bypass mode is grabbing the wrong image bits, but ReadDax gets the right ones…