3:00 P – 8:00 P, 9:30 P – 11:15 P
Kinetic modeling
- Discussion with Jeff
- sent text books (see email)
- Lent library book (Feller) to Jeff
- should write text together?
OligoPaint Probe making
- spin down ethanol precipitating samples of probe cut from gel
- Also spin down PPT protected and non-protected samples
- Resuspend in 20 uL ddH2O
Problem solved?
- Used short forward instead of short reverse primer in making B/Y/K/G probes.
- Repeating at 2 mL scale with correct probes. Will test on gel tomorrow
Restart Ubx, AbdA. AbdB
- 4 mL scale each
- new dNTPs, fresh Taq,
- In total ~17 mL of PCR (24 + 33)
Data storage:
12 TB system:
Mediasonic HFR2-ASU3S2 SmartShield 4 Bay External Hard Drive Enclosure
Amazon
4x
Western Digital Caviar Green 3 TB SATA III 64 MB Cache Bare/OEM Desktop Hard Drive – WD30EZRX
Amazon
$ 130 each
Total: $713.00
Capacity: 12 Tb
RAID Capacity: 9 Tb
24 TB system:
Mediasonic H8R2-SU3S2 ProRaid 8 Bay External Hard Drive Enclosure – USB 3.0 & eSATA (Black)
Amazon
$350
8x
Western Digital Caviar Green 3 TB SATA III 64 MB Cache Bare/OEM Desktop Hard Drive – WD30EZRX
Amazon
$ 130 each
Total: $ 1398
Total storage: 24 Tb (58.25 per Tb) .
RAID Capacity: 21 Tb