10:00 A – 8:00 P
Ph-FLAG analysis / clustering
- Blocking total counts per cell (after applying mask) results in good comparison. Currently 50-300K: window probably a bit large but need to get the cell numbers up.
- Ph wt data: Buffer didn’t last too well? some cells look over-dried maybe? Should repeat.
- Ph-M flag: two data sets look good
- Ph-wt-flag: didn’t image enough frames to make quantitative comparison to previous data set (50K vs 120K frames).
Ph-FLAG planning
- reagents for Ajaz.
- set up to stain mutant, wt, and s2 cells all with wt Ph. use 488 to mark transformants.
- wt-ph embryo staining
STORM
- what’s the additional slider under the objective do? When did this get added to the beam path?
- All quadview images from all color channels are getting mapped to the 647 corner. Why? This makes it very difficult to take bead movies appropriately…
- spent 2 hours so far trying to get 750 to image appropriately