8:00 A – 7:30 P, 8:3 0P – 11:50 P
Presentation
- Finalizing talk for Wu group meeting
- most interested in large scale structure
- suggestion: hox-gene loci in mouse ES cells (larger regions easier to get structure)
- how about the Duboule Archipelago?
- contacted Zyagen about purchasing tissue sections. Contacted Ting about potential collaborators or mouse limb bud / hox experts in Boston area? Maybe I should just write to Bickmore, though shipping mouse samples in paraffin or frozen over international borders might not be easy.
STORM
- en-embryo labeling FAILED! should have checked this slide earlier.
- should stain in parallel with cells. No idea why this failed. Did not record staining details in notebook. bad.
- STORM imaging BLACK DNA regions in PDMS well setup. calibration cell has excellent switching. slightly concerned that buffer might be running down fast because brightness of individual fluorphores seems to go down later in the image, but not sure — maybe it is only bleaching
- settings = 45% laser power, 200 mW set.