10:20 A – 11:50 P
Coding
- Fixed bug in ReadDaxBeta (missing factor of 2 in converting bits and bytes).
- wrote script to make a movie of ‘building a storm image’ (
PlotDax2Bin.m). Currently in AlistairBeta.
Presentation prep for SDB
- working on slides: primarily assembly of STORM image
Other
- refreshing HPLC start-up
- help Brian get set up with Bryan to HPLC LNA probes.
STORM
- K DNA switching looks bright right through the end of image collection.
- no sign of air bubbles or fluid loss from PDMS well
STORM analysis
- set up spot counting analysis (DaoSTORM) for Ph-wt in Ph-FLAG background, Ph-M-FLAG background, and S2 cells.
- set up analysis of black DNA locus. Much better contrast than in Ph stains, not sure why.
- STORM yellow DNA. Lots of switching, still ,5% laser power at 125K frames. Stopped at that.
Tissue sections
- Reference on NBF fixation of tissue (neutral buffered formalin), used termonology used by Zyagen on their tissue sample preps.
- follow up with Zyagen about cost and block size.