9:50 A – 5:30 P
Banff
- finish draft schedule
- upload draft schedule to website
- finish activities sheet
- send participant email list, activities sheet, draft schedule, and notes about filming talks and requesting titles to Andrew.
Planning with Jeff for next week
Experiments on deck
- Setup next week to deep sequence library
- troubleshoot exon selection next week
- RNA seq cells
troubleshooting RNA isolation
- substantial nucleotide fraction left even after ethanol precipitation. Better to compare levels by gel than by nano-drop.
Probe making
- finish in vitro transcription reactions, check on gel
- ran 1uL of 20 uM cy5 labeled primer next to ladder.
- very poor incorporation: very strong cy5 band below the RNA bands.
- cy5 labeled primer runs faster than unlabeled cy5.
- usual 2% ultrapure agarose gel run for 10 min in sodium borax with Ultra low range ladder on left, bright ladder band is 50bp.
STORM
- Finish O/N run of slide 1 round 2, Bxc. Transfer data
- O/N STORM analysis of Gdc data still running
Chromatin
- probe BLAST ran O/N with original 25mer oligo list, not pruned primer converted list.
- rerunning.
- fixing bugs, converting to functions, making sure results against separate genomes are documented in separate fasta files.
To Do
- Pc Psc analysis!
- Review!