10:30a – 10:30p
Helping Hao align FRET
- Wrote
SplitQVdax.m - SplitDaxFRET_130817
- Note: splitting large dax files is slow and memory intensive. Even 2000 frames takes a few gigs in the memory map approach.
- (10:30-2:30). Will need to help get setup with local version of matlab-storm.
Review
- Working on reference formatting
BIRS
- layout draft version of schedule and speaker organization
- send draft to co-organizers
Probe making
- column cleanup DNA
- RNA synthesis
- setup in vitro transcription reactions with 7.5 uL of template (~150 ng/uL).
- samples 1-3 are Ubx, AbdA, AbdB with modified UTPs
- samples 4-6 have unmodified nucleic acids.
- run at 37C for 16hrs (2pm tomorrow)
STORM
- imaging high quality cross-section of germ-band retracted embryo for BX-C spots. not good switching and high background. Really should get the BX_C activators. Dots prior to attempting STORM look bright and clear.