for each sublib (50 uL reaction)
- 19 ddH2O
- 5 uL 5 uM common
- 1 uL of 1:10 diluted library
- 25 uL Phusion master
- 0.25 uL of 200 uM T7 lib specific primer
master mix (83x)
- 1577 uL ddH2O
- 415 uL 5uM common
- 8 uL library
- 2075 uL Phusion Maser Mix
-
82 sample gel
- Wells not deep enough to get a good focused image
- A1-12 and B1-12 alternate the first 24 wells after the ladder (due to width of multi-channel pipetter)
- C1-12 and D1-12 alternate the next 24 wells at the top
- E1-12 and F-12 alternate the first 24 wells on the bottom, followed by G1-10.
- G7 failed again, G1 had difficulty but looks like it may have amplified this time.
