8:00a – 7:15p, 10:15p-11:00p
Probe making
- set up in vitro Tx reactions
- run new gel with ladder: G1 and G7 look blank, others look good (a bit troubling, since G7 nanodrops at ~75ng/uL even though G1 goes blank).
- Full library PCR and test gel (see notes)
discussion for improvement
- Try RNA beads to purify RNA on magnet (have a plate format as well. Should work in small volumes)
- Try skipping RNA purification all together (do both ways and compare product)
- Compare SuperscriptII and SuperscriptIII side by side.
Other
- look into Burroughs Welcome pre-application