8:15a – 7:00p
Multi-stain analysis
- try crosslinking 415 beads to fresh, unfixed cells
- initial beads somewhat clumped and extremely bright, especially under high 405 illumination (or minimal weak 488 illumination). Beads do successfully blead into 647 channel at moderate brightness in quadview mode at 5Hz. Probably still visible at 60Hz.
- Took cells through the rest of ‘3d-FISH’ prehybe treatment. We’ll see if there are still beads at the end.
Cell culture
- passage clone8 cells
- check Kc167 cells.
- passage Kc167 cells to new 75 cm^2 flask
- Create new frozen stocks of Kc167 cells (7 vials) and Clone8 cells (10 vials). Now slow-freezing in the -80C.
- Move these to LN2 storage tomorrow.
Probe making
- Library 2 plate scale PCR clean-up
- Nanodrop from some samples:
Coding
- helping Hao use matlab-storm functions for FRET analysis
Planning
- ordered Zymo CC-5 plates and RNA 100 columns