Thursday 09/19/13

9:00a – 7:45p, 10:00p – 11:00p

Writing

Referee work

  • working remotely (9a-12p)
  • write up comments

BJ review

  • Goal: Trim down BJ review by 3 of 16 pages
  • started trimming from beginning. 1 full page shorter.

mRNA indexing

  • human genome 22917 primers kept at 13 or less homology of 25 bp. (After parallel processing analysis)
  • Patterns of Drosophila genes in 100 kb region
  • CG11127

    CG11127

    p47

    p47

    Aldh-III

    Aldh-III

    CG1620

    CG1620

    CG1621

    CG1621

    dpa

    dpa

    az2

    az2

    CG1602

    CG1602

    CG1600

    CG1600

    CG1598

    CG1598

    G8_poordetection

    G8_poordetection

    G4_STORM_sample

    G4_STORM_sample

    G8_undersampled

    G8_undersampled

    G6_tubregion_sample

    G6_tubregion_sample

Review recent STORM data

  • some samples, G4 G6 and G8:
  • G4_STORM_sample

G8_undersampled

G6_tubregion_sample
* G8 might be good to repeat — low intensity, sparse samples:
* G8_poordetection

STORM

  • attempting new STORM of G8
  • switching much better in sample 1, buffer is better
  • however, overall staining very weak, not obvious in all cells and bleaches fast.
  • probably need to make probe at higher concentration.

Coding

  • helping Hao with spot-counting code
  • debugged image alignment
  • saved new version
  • Couldn’t find fast-forward fork
  • did work to submit pull request and merge that, after swapping the compare branches.

Domestic

  • prep for NH trip
  • pick up stove, fuel, sleeping bag, pad, pot.
  • check for iodine

Domestic

  • submit reimbursement paperwork for Banff (charge to fellowship funds)
  • contact Matt about Mexico (charge to fellowship funds)
  • contact Matt about healthcare reimbursements
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