Wednesday 09/18/13

9:15a – 11:30p

Writing

• working on BIRS workshop report (9:15a- 12p)

Probe making / training with Bogdan

• test PCR from yesterday
• common primer concentration was a bit low
• gel didn’t fully dissolve, might have affected gel running:
• 
• Repeat PCRs. Repeat Gel. Looks good. Negative control is clean
• 
• nanodrop concentrations

STORM

• BXC-embryo imaging
• good staining, good morphology, some very clear anterior / posterior differences. More subtle early embryo differences.
• hand imaged ~15 embryos

Coding

• improving massive primer design analysis — needs to be paralyzed for BLAST to large genomes.
• code is BuildOrthogPrimers_130919.m running overnight now on HumanData. Uses new function BLASTbatch.m