Friday 09/27/13

9:00a – 4:30p

Multiday Goals

• Write letter to Ting and company
• Design D mel. gene library
• complete ANT-C stains (G1-G5) (need G1 probe to complete).

Probe Making

PCR cleanups:

• Large Regions
  1. D11 100 kb percicentric Green
  2. D12 385 kb Yellow
  3. E01 200 kb Green
  4. E8 75kb Red
  5. E10 98 kb pure yellow
  6. E12 630 kb Green
  7. F1 BXC_K_left, 125kb pure K
  8. F3 Ubx through bxd
  9. F4 AbdA through iab4
  10. F5 iab5 through AbdB
  11. F7 Bxc_K right 240 kb solid K
  12. F10 478 kb Black
  13. F11 ANTCleftBlack 180 kb
  14. G9 76 kb Blue near ANTC
  15. G10 425 kb black region
• small regions (which failed in first round)
  1. E03 BLUE 13 KB (no genes, good PC/Psc)
  2. E04 BLUE 10 Kb (flanked by strong PolII)
  3. F08 RED 7 kb region next to black 1, tRNA locus
  4. G07 AlphaTub84 (5 kb yellow)
  5. G01
  6. G02 (positive control)

Presentation

• collecting “on-hold” Pc results into presentation for Ting & Fred.

Communication

• Accept invitation to speak at Rowland Center
• Accept invitation to speak at Stowers Institute
• Accept invitation to speak at Pairing Meeting