Monday 09/30/13

Posted on September 30, 2013 by admin

10:00a – 11:00a, 6:20p – 9:50p

Oligo cleanup and concentration measurements for short-RNAs (pure and raw)

  • (1) E02 13 kb green (between R/Y)
  • (2) E06 Epc (12 kb YELLOW)
  • (3) E07 Tou (15 kb YELLOW)
  • (4) E09 RED 8 kb intronic tRNA locus, in between blue
  • (5) F02 BXC_Y_left (15 kb)
  • (6) F09 BLACK 18 kb region next to RED1
  • (7) F12 Taf1_(17kb yellow/green)
  • (8) G01 LabRegion_(Lab_Zen2) [83 kb but missing piece of ANT-C]
  • see Thursday for gel.

Concentrations

  • (all had 300 pmol of 40bp 405-primer. Would be 250 ng/uL in 15uL if it was all primer. Would be ~800 ng/uL if all became probe.)
    shortProbes_PvR
    shortProbes_plots

in situs

  • samples E2, E9 and G1 using raw-RNA reactions.
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