9:30a-4:30p, 7:00p-12:00a
Goals
- Figures for Ajaz!
- Work on Banff report
- Start presentation for Stowers
- order Illumina MiSeq components
- Order NEB primers
- PCR
Overnight power failures?
- Tuck auto-restarted in night. Overnight analysis aborted/lost
- Monet auto-restarted in night. Overnight analysis aborted/lost
- IR laser turned off in middle of STORM2 run (image 5) !?
- 488 laser turned off in middle of STORM2 run
- power surge partial power loss to Chemistry at 1am (only 1 hour after I left. Damn it).
- restart data-transfer
- Graham offers his scope time to allow recovery of my run. Thankyou!
Biophys J review
- Final re-read and revise, sent back to editors via email
Ph Project
- Working on Ph Figures
- Working on Ph model
Cell culture
- new media
- fix new plate of cells (3 mL straight culture each), easily homogeneous coating (probably 2x)
Library validation
- set up 9 cycle PCR