Friday 10/04/13

9:30a-4:30p, 7:00p-12:00a

Goals

  • Figures for Ajaz!
  • Work on Banff report
  • Start presentation for Stowers
  • order Illumina MiSeq components
  • Order NEB primers
  • PCR

Overnight power failures?

  • Tuck auto-restarted in night. Overnight analysis aborted/lost
  • Monet auto-restarted in night. Overnight analysis aborted/lost
  • IR laser turned off in middle of STORM2 run (image 5) !?
  • 488 laser turned off in middle of STORM2 run
  • power surge partial power loss to Chemistry at 1am (only 1 hour after I left. Damn it).
  • restart data-transfer
  • Graham offers his scope time to allow recovery of my run. Thankyou!

Biophys J review

  • Final re-read and revise, sent back to editors via email

Ph Project

  • Working on Ph Figures
  • Working on Ph model

Cell culture

  • new media
  • fix new plate of cells (3 mL straight culture each), easily homogeneous coating (probably 2x)

Library validation

  • set up 9 cycle PCR
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