Wednesday 11/27/13

Goals

• Sequencing analysis
  • plots of coverage uniformity per probe set
  • plots of failed probe vs. purity using new indexed method (or maybe just a length cut-off)
• Ph Project
  • restart simulations
• Genomics
  • start building library 4 (on Monet)
  • start building library 5 (on Odyssey)
• Chromatin imaging
  • Start STORM fitting new regions:
  • (D08 and D10 from STORM4, D11 and F6 from STORM2)
  • ChromatinCropper of new regions
• Chromatin Library 3
  • cut up G10 into subsections
  • start building library

Lab Work

• Finish O/N STORM on STORM2 of D11
• move Kc cells from -20C to LN2 (11am)

Computer work

• Relaunched Ph simulations with capping model parameters
• Building Fasta file of all transcribed genes for project2 cell line.