Friday 12/13/13

10:45a – 12:15a

Notebook

• backed up notebook
• updated wordpress

Z-calibration

• cross correlation doesn’t improve curve alignment visibly — curves are fundamentally a bit different
• no spatial structure evident
  

Ph Project

• reviewing simulation results
• saved as simulationName_2.eps to file

Test new stains

• at higher concentration, probes work great. Will need to make more though, total yield here was actually quite poor then. Try splitting RT into smaller reactions.
• Beads at 1:1000 this time way too dense. Single beads look fine on glass, but on cells this time the beads are coated too dense to separate one from another.
• try again at high concentration to do the 750 labeling. Try D12-D12 750 + 647.

probe making

• spec’d concentrations of primers but otherwise failed to start PCRs

STORM analysis

• worked through black regions, added images of 4 black regions (10, 18, 180, and 425 kb) to presentation
• helping Guipeng get DaoSTORM working
• need to sync up matlab-storm versions, push to master run clean install. (could create dummy user on Tuck to test?)

Project 2

• Ran a variety of error analysis with Hao
• Computed error over time — confirmed no detectable / statistically significant increase.