9:15a – 12:15a
Plans for week
- probe making
- troubleshooting probe smears. Give Jeff some library material.
- make new probes (F01, F02, F07) + control + some of Jeff’s probes
- test different RNase inhibitors?
- Order Library 3
- trim probe list (remove some big black and redundant BX-C)
- design new primer set
- Ph project
- image psc cells for Ajaz
Meetings
Analyzing Chromatin regions
- see post
- working on summary statistics for all regions
Ph project
- STORM imaging of Psc and PhM-flag (double stained)
- sorta sparse slide.
- Should really co-stain lamina, with the nuclear compartment thing going on its not so obvious what’s nucleus and whats cytoplasm in all these cells.