9:45a – 12:15a

Goals Today

• Rewrite ColorIntervalJumpGap.m Function to allow different gaps for different chromatin types.
• Select new Red regions

Chromatin Project

analysis

• Designing some new regions: notes
• Writing up summary of chromatin regions thus far: see protected notes.
• Let’s try to make a mini-lab meeting like presentation for my meeting with XZ on Tuesday, and plan to order Library 3 between the end of that meeting (if no changes need to be made) and the end of that week (if we change priorities for what goes in there).
• Let’s aim to have all the potential regions folded and sorted with their primers before then.

STORM

• imaging F03-647 F04-750.
• calibration image looked pretty good
• 750 background very low, dots pretty faint though / fast bleaching. I’ve gotten much brighter 750 in the past. Might want to increase 750 probe conc. slightly.
• switching not very bright (2.5 uL BME + 5 uL COT). Could use brighter dyes. Maybe try with MEA tomorrow. That’s my only day-time ‘scope time this week. Can try with same coverslip.

Project 2, code organization

• discuss public vs. private reps
• discuss folder organization

Ph Project

• Start analysis of Psc PhM data