10:00a – 11:30p
PH project
Meetings
- Finalize slides
- discussion with XZ
- Add effect on cluster count instead of effect on cluster size.
Updates
- working on figure for the effects on cluster count
- added cluster counts vs. cluster diameter
- some further explorations in concentration effects:
Seminar
- Bodo Stern Seminar on getting published from the Editor’s perspective (former Chief Editor of Cell)
- see notes
Chromatin Project
Deep sequencing prep
- samples 1-8: A01-08 + B02-09
- samples 9-16: A05-12 + B03-B10
- 5 rounds of PCR
- ladder + samples 1-14
Final prep
- ran 2 more rounds of PCR for samples 1-8. (doesn’t seem to have made much difference?, probably could have done another 5, these were pretty dim).
- Gel extracted all samples
- Centrifuge broke (all 3 lights flashing). Samples swallowed (~11:30pm)
- Next morning, (11:00a) extracted samples from centrifuge with emergency release at bottom (see manual), and finished eluting samples on alternative centrifuge. (hopefully these are still good. I guess we’ll know during the RT PCR)
Sample key
- Lib2-x1
- Lib2-x2
- Lib2-x1 v2.
- HaoLib4 E1
- HaoLib4 E3
- D12-P1
- G09-P1
- F11-P3 Nc
- Fll-P3 12-18
- F11-P3
- F12 P3 NC
- F12 P3 1580
- Hao L3-uni + L3 i1R
- Hao L3-uni + L3 i2R
- Hao L4 E1F + E1R
- Hao L4 E3F + E3R