Flash Memory: photochemical imprinting of neuronal action potentionals onto a microbial rhodopsin (Cohen Lab). JACS Jan 2014.
High Res confocal microscopy (not STORM/PALM/SMACAM)
- Two PRL papers
- High-resolution confocal microscopy by saturated excitation of fluorescence. Fujita et al, PRL, November 2007.
- Measurement of a saturated emission of optical radiation from gold nanoparticles: Application to an ultrahigh resolution microscope.
Chu et al, PRL, January 2014.
Intro, review the 2007 work
- principle: saturation (more laser less response at some point). – this is a non-linearity we can exploit to improve resolution
- in spatial domain this makes the psf larger
- in the Fourier domain you get multiple peaks (‘overtone’), more power in these peaks as you increase intensity.
- find the positions in the image that get overtone are narrow versions of the other.
2014
- saturate the scattering of gold particles (instead of fluorescence)
- also look at higher modes, get scattering.
Questions
- compare to saturated nonlinear SIM?
- similar prinicple