Monday 02/10/14

Posted on February 10, 2014 by admin

9:30a – 11:50p

Meetings

  • Group meeting (see notes)
  • Journal club
  • team project meeting

Literature

  • interesting perspective: http://www.sciencemag.org/content/343/6171/596.full
  • Pauli’s paper: discovering new developmental signalling peptide (Science)

Chromatin

Data analysis

  • Analyzed G01-G02 up to image 23
  • start (re-)analyzing F03.
  • F03 data analyzed up through image 16 (34 spots, plenty more to image).
  • F04 data analyzed up to image 22 (33 spots, plenty more to image)
  • F05 data — beads not as bright as bead-parameter settings. Rerunning bead analysis.

Cell prep

  • passage cells
  • plate cells for new staining
  • prepping cells
  • New stains: G01 + G02 + G03 (all 647)
  • F01 + F02 (all 647)

Seq Prep

  • 16 samples, 1:50 dilution, then 1:20 dilution. 4:196, 5:95
  • Column 1: rows 1-8 (A-H): 1:1000 dilutions of samples 1-8
  • Column 2: rows 1-8 (A-H): 1:1000 dilutions of samples 1-8 Replicate
  • Column 3: rows A-H: 1:1000 dilutions of samples 9-16
  • Column 4: rows A-H: 1:1000 dilutions of samples 9-16 Replicate
  • Column 5: 1:10K dilution of samples 1-8
  • Column 6: 1:10K dilution of samples 1-8 Replicate
  • Column 7: 1:10K dilutions of samples 9-16
  • Column 8: 1:10K dilutions of samples 9-16 Replicate
  • Column 9: old library, Replicates in A, B and C, 1:1000 dilution. Replicates D,E,F 1:10K
  • Column 10: DNA standards 1-6
  • Column 11: DNA standards 1-6 replicate
  • 4 ul of sample (diluted sample or straight standard) + 6 uL of qPCR master mix.

Ph Project

Attempting STORM + conv DNA FISH

  • prepping cells for in situs
  • accidently took S2 + 2 WTs (wanted S2 + WT + M). Oh well, let’s see if this works at all for WT anyway
  • running RNase digestion (9:45p)
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