(sick, working from home)
Library3 prep continues
- running primer finding
- adding more filtering
- do a direct comparison on final 5 basepairs,
- reject all perfect matches, keep only unique ones.
- reran a few times to debug
- raising T min to 74C (76 gives too few primers)
- this dramatically reduces the number of primers before BLAST.
- BLAST runs way faster with thousands rather than 10s of thousands of primers
- interestingly doesn’t dramatically reduce final primers out
- Numbering is a bit annoying from mixed library runs
- rerunning oligoArray overnight with new Lib3Table with all genes numbered
Ph Project
- write to Ajaz about the FISH data
- Ajaz also wants to know about the Psc data