Tag Archives: Library3

Monday 02/24/14

Posted on February 24, 2014 by admin

(sick, working from home) Library3 prep continues running primer finding adding more filtering do a direct comparison on final 5 basepairs, reject all perfect matches, keep only unique ones. reran a few times to debug raising T min to 74C … Continue reading →

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chromatin library organization and design

Posted on January 14, 2014 by admin

Introduction / Motivation Current library 3 design is still missing some key regions we would like to investigate very soon. For example there is no concerted attempt to bring red regions back into the fold. Preliminary observations of red regions … Continue reading →

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Friday, 01/10/14

Posted on January 10, 2014 by admin

9:45a – 6:15p STORM imaging – multi-color chromatin O/N imaging of F5 F6 lost focus very early in night. O/N imaging buffer also did not survive well Re-imaging F11 F12 new freshly made Glox 2.5 uL BME + 5 uL … Continue reading →

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Friday 01/03/2014

Posted on January 3, 2014 by admin

remotely 10:30a – 11:30a (snowstorm) Winterize bike: 11:30a – 2:00p lab: 2:00p – 9:00p Goals switch road tires for mountains for better stability in the snow Design new primer collection for chromatin library 3. Pick more regions for multi-color experiments: … Continue reading →

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Thursday 01/02/2014

Posted on January 2, 2014 by admin

9:30a – 9:00p Ph project Finish O/N STORM run Take IR bead images (slide is also getting clumpy. Maybe we need to stick these down with EDAC. I think making slides for long term storage with Glox is also a … Continue reading →

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