11:00a
Maintenance stuff
- moved 2013-10-07_F09 and 2013-10-09_F01V2 from proRAID to ProBox1 to make room for more Ph datasets on ProRAID1
- deleted these files from ProRAID. Now moving all Ph datasets from ProRAID2 onto ProRAID1 (another 1.3 TB. some of these could use compression still).
- automatic analyze on arrival didn’t reset wait-times, consequently failed to keep launching analysis throughout the night. Fix and start running.
Robert Singer Visit
- meet at Balskus Lab at 12:10p
Chromatin Project
- Set up PCRs
- 25 uL Phusion + 20 uL ddH2O + 1 uL 1:50 library + 2.5 uL 10uM Forward + 2.5 uL 10uM ReverseT7
- regions selected
- C01 chr2L:10203488-10443191__Y_240kb_TAD_G-gaps
- C02 chrX:16351360-16440669__K_90kb_Disco_TAD
- C03 chr3R:17205633-17399201__BLUE_200kb_lbe-Tin_cluster
- C04 chrX:15953918-16160086__BLUE_200kb_disco
- C05 chr3R:3953538-4054361__BLUE_100kb_grn
- C06 chr2R:1593366-1640664__BLUE_47kb
- C07 chr3R:11833445-11870730__BLUE_37kb_pnr_GATAe
- C08 chr2R:5080631-5092410__BLUE_12kb
- PCR failed (Gel failed?)