10:00a – 1:00a
- Data for Jeff
- Density distributions for over sequence for template and probe from my library
- Cumulative dist plots for probe abundances from my library
- Insertion/deletion/mutation error plots as a function of bp for template and probe
- New figure for Ting
- Quantification of signal per spot as a function of labeling scheme, with images of each.
- Quantification of background per cell as a function of labeling scheme, with images of each.
- Quantification of on-target to off-target probe binding frequency.
- Upgrade Cajal to Windows Server 2012
- upgrade took all day and failed
- I think I learned a lot about how Windows Server 2012 has changed.
- see posts on subject.
- STORM4 imaging
- serious issues with camera alignment
- serious issues with quadview alignment
- probably issues with back-beam alignment secondary to camera misalignment, can’t diagnose.
- laser connectivity issues to GUI?! GUI stopped talking to 642 and 561 laser. not good not seen this before.
- unable to resolve all issues in time for an overnight run.