10:15a – 11:00p
- XZ wants to meet at 3P, need to make slides (for project 2)
- see more project 2 protected notes.
- sample E03 (previously stained beautifully) failed to work on new cells.
- Not a simple omission of something in sample prep.
- Old C01 stains look great. Not a buffer issue or other microscope issue.
- old cells (previously stained with C08, dim stain, should also washout during hybe). Stain with C1, 1.5 uL.
- new cells + RNase, Stain with C1 1.5 uL
- new cells – RNase, Stain with C1 1.5 uL
- dilute everything in old “hybe mix” (same buffer, different aliquot than used yesterday).
- prehybe in new 2X SCCT + 50% formamide made yesterday.