10:30a – 4:00p
New probes to make:
- B10-B12 (last of the yellows)
- C09-C12 (last blue, first 2 blacks)
- D01-D07 (all the end-to-end blacks)
- [D08-D12 are Bogdan’s internal blacks]
master mix
- 525 polymerase (25*21)
- 420 ddH2O
- 21 of Lib3 (1:50 dilution)
- 45 uL to each well
- 2.5 uL of F-primer,
- 2.5 uL R-primer