10:00am – 7:00pm
Goals
- Call Otto’s order pizza for lab meeting (done)
- Finish lcPCR and cleanup (done)
- Set up T7 reactions (done)
- post-fix Ajaz samples to try immuno-FISH again (done)
- stain cells (done)
- finish letter and reply to Nicole about collaboration (done)
- review and send fellowship progress report to XZ (done)
- Review manuscript draft for Max (post-poned)
Making probes:
probes
- B10-B12 (last of the yellows)
- C09-C12 (last blue, first 2 blacks)
- D01-D07 (all the end-to-end blacks)
- [D08-D12 are Bogdan’s internal blacks]
Progress
- Ran QPCRs
- Ran PCR cleanup
- running 30 uL T7 reactions O/N
Cell Staining
- post-fix in 1% GA 4% PFA 1 hr.
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- S2 anti-PH
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- PHwt anti-flag
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- PHmut anti-flag
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- washed with PBS, then 2x SSC, then prehybe.
- Stain BXC and ANTC
- G02-P2-cy3
- F05-P1-405 (old sample) + S1-488
New Probes to stain
- E11 + E12 + F01 (G06/alphaTub locus)
- E11
- E12
- F01 (alpha Tub itself)
- B08
- B09
- all with P1-405 + S1-A647
Ph Project
- remade figure for neg controls (separated channels, matched cropping boxes)
- sent figures for neg controls to Ajaz
- review figures
- finish draft letter for Nicole and sent back