10:30 am – 3:00 pm, 6:00 pm – 7:00 pm, 8:30 pm – 11:30 pm
STORM
- finish imaging C11
Cell prep
- passage newly plated cells to 75cm2 flask
- fix cells, prep cells for staining
Probe making
- run RT PCR for D08 – D12 + neg cntrl
- run PCR cleanup
- run IVT reaction
Chromatin Analysis
- Analyzed B10 up through image 23
- Analyzed B11 except last 3 images (7, 8, 9)
- Analyzed B12 through image 4
- Analyzed F11toF01 through image 26 (50+ spots)
- Analyzing F01 nearly same size as F11 to F01 ( through image 31, 43 spts)
- Analyzing E12 much smaller than F01 (despite 2x more DNA, 10kb vs 5 kb),