10:45 am – 9:55 pm
Teaching
- training Bogdan on Cryo
- cutting mouse brain. Target size 1um
- initial cutting temperature = -55 C
- sections look reasonable with DAPI.
- collecting sections for imaging
- teaching proper clean up procedure
- New supplies to get
- backup small perfect loop.
- brushes
Chromatin
Sequential staining
- S3toe5 failed quality control, won’t ship until tomorrow
- S1toe5 shipped last night, should be here to test with
- can I do S3 after S1? This actually has 15 bp of homology because of the shared 10 bp.
- imaging BXC-S1. At least these look like some good BX-C images.
Internal scaling
- selecting and cropping sample images for figures.