Tag Archives: images

Friday 11/16/12

Posted on November 16, 2012 by admin

9:45 A – 7:00P, 8:00P – 10:30P Making summary images of SCM data. Reconcentrating / Precipitating probe prior to nicking reaction. Combined into 2 tubes. Dissolving pellets at 37 O/N.  (waiting for Nicking enzyme to continue). Nicking enzyme arrived Brian … Continue reading →

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Protected: SCM summary

Posted on November 16, 2012 by admin

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issues with non-blinking weak spots

Posted on November 15, 2012 by admin

The very bright, round foci clearly do not correspond to objects in the conventional image and are probably all artificats from non-switching foci. These dots start off at moderate single molecule brightness (can’t eliminate them just using higher threshold cutoff … Continue reading →

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Testing small oligopaint probes

Posted on November 10, 2012 by admin

First small test probe Reagents The primer  has a 5′ TYE665 dye. Both primers are at 200 µM (200 pmoles/µl).  template was at 10 ng/µl. A look at the genomic region Zoom in on region   Question for Ting /Brian … Continue reading →

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in situ update

Posted on October 28, 2012 by admin
[spreadsheet 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE 602 302 sheet=8]
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observations on Insulator organization

Posted on October 16, 2012 by admin

CTCF sites are TF rich: (en locus) CTCF sites are TF rich ~HOT DNAs?  (though not as strong DNA-hypersensitive regions).  rich ~HOT DNAs?  (though not as strong DNA-hypersensitive regions). Do these insulators co-localize (most insulators HiC/3C together) Coerces 2012 MolCell chromatin domain boundaries … Continue reading →

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Protected: Analysis of En locus k27me3

Posted on October 15, 2012 by admin

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Protected: Pc and K27me3 dual labeled first analysis

Posted on October 15, 2012 by admin

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Protected: K27me3 density

Posted on October 14, 2012 by admin

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Protected: polycomb silencing super-resolution images at ANT-C, BX-C and En loci

Posted on October 10, 2012 by admin

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