Tag Archives: images

Wednesday 01/25/12

Posted on January 25, 2012 by admin

10:20 A Analyzing confocal images from testing antibodies WGA-488, H3K27me3-Cy3B (red), H3K4me3-Cy5 (blue) running LSM files through imviewer install TeXMaker on desktop working on fellowship applications BIRS proposal for 2013 5 day workshop rejected.  Contacted co-organizers to re-stratagize. Writing imviewer … Continue reading →

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Saturday 01/21/12

Posted on January 21, 2012 by admin

8:45 A — 2:00 P in situs moved through resin, dense collection of embryos 10 embedding capsules of embryos made for each of the 4 in situ tubes (+4 of tube 1 by KE for practice). in situ embryos baking … Continue reading →

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Friday 01/20/12

Posted on January 20, 2012 by admin

10:10 A -11:00 P labeling primary anti-histone antibodies (see Alistair_STORM_dyes log): pan-H3, H3K4me3, H3K27me3, and H3K27ac.  Need to filter Ac and K27 through column before binding.  Also filtered H3 and K4 — dilutes sample sufficiently to allow for multiple subsequent … Continue reading →

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3D histone views

Posted on January 20, 2012 by admin

3D reassembly of microsection stack of STORM images of individual nuclei with pan-histone marker.  Some alignment issues still, but worth a comparison

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Tuesday 01-17-12

Posted on January 17, 2012 by admin

9:30A – 7:00 P in situs day 1 + training KE Flip Fly cages 10:45 A Flip collection cages: 12:45 P 3:45PM fix embryos (2-5hr MP08 collection) working on figure for fellowship app

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Protected: shadow investigation

Posted on February 3, 2011 by admin

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Protected: hb enhancer modeling cont…

Posted on January 19, 2011 by admin

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