Tag Archives: coding

Friday 07/06/12

Posted on July 6, 2012 by admin

10:10 A – 7:30P, 9:00P -11:15P Flip fly cages, 10:10A Revisions of dynamics paper, revised, updated to v7. compare to NM requirements.  Version and comparison sent back to ML.  May need to combine figures and prune text. Take embryos out … Continue reading →

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Thursday 06/21/12

Posted on June 21, 2012 by admin

2:00P — 8:30P Calibrating new bead imaging: 1:20 then 1:500 540 beads + 1:500 Q-dots-705.  Too dense. Dilute a further 1:500.  Q dots good density.  Blinky, excited best by 405 or 488.  Look good in 647, not really visible in 750 … Continue reading →

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Wednesday 06/20/12

Posted on June 20, 2012 by admin

10:50 A – 6:30P ordered 0.04 um Fluospheres (540 + 715).  large fluospheres give unusable 3D maps No 715 Fluospheres, ordered 20-nm 705 quantum dots Fixed error in Z-cal convergence.  Modified convergence measure to use both x and y data. … Continue reading →

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Tuesday 06/12/12

Posted on June 13, 2012 by admin

10:00 A-5:00P, 9:30P – 12:00A Flip collection cages, 10:30A still working on z-calibration bug.  updated write_minimal_dax_ini file seems to be the only script that won’t run correctly.  Putting max_displacement = 1 causes errors in otherwise functional script.  May need to … Continue reading →

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Sunday 06/10/12

Posted on June 10, 2012 by admin

4:00P – 7:30 P old-version of auto z-cal converges effectively on old and new data. Start making slides for meeting with Xiaowei next week. New probes to make: lab, scr, bxd, mir-10, Ama, CG5220 + CG3995, CG10324 + CG 10326, … Continue reading →

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Wednesday 06/06/12

Posted on June 6, 2012 by admin

10:30 A – 1:00P, 4:00P-7:00 P troubleshooting 3d warping (see post) Final problem looks like artifact from trying to get 3D fitting data from 2D planes of beads.  Try to shoot more beads tomorrow. in situs day 2 with KE … Continue reading →

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chromatic warp issues

Posted on June 6, 2012 by admin

warped image blurs out certain channels.  here 561 gets stretched along x. In the K27/K4 dataset 750 is dramatically stretched along x-y in a correlated fashion. Distortions get worse using higher order polynomial (e.g. 4th order).   Distortions are no … Continue reading →

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Tuesday 06/05/12

Posted on June 5, 2012 by admin

10:00A – 8:00P in situs day 1 with KE: new large cage of Pc-GFP and Yw Flip 3x Mat-Gal4 into new bottles.  Moved to 25C. troubleshooting chromatic warp issues in new vector storm code.

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Monday 06/04/12

Posted on June 4, 2012 by admin

10:00 A-12:00P, 5:30P-7:30P, 9:30P-10:30P Brian group meeting Katherine Ebright starting summer program in lab. working on vector image analysis for K27me3, K4me3

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Wednesday 05/30/12

Posted on May 30, 2012 by admin

12:00 P -8:00P clean and coat 2 racks of small coverslips confocal analysis of embedded oligo-paint sample.  Embryos not detectable. sectioning of Su(Z)12-750 K27me3-647 + Dm0-750,647 working on new vector version STORM analysis code.

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