Tag Archives: STORM

Tuesday 02/28/12

Posted on February 28, 2012 by admin

9:45 A – 12:45 A Evaluating 3D fitting: section joins do not look smooth Wrote new script (list2tif3d_weq.m) which distributes samples uniformly in z.  Samples near the focal plane are best resolved and can have higher z-resolution. Current approach still … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Tuesday 02/28/12

Protected: Super-resolution imaging of PcG bodies

Posted on February 27, 2012 by admin

There is no excerpt because this is a protected post.

Posted in Microscopy | Tagged , , | Comments Off on Protected: Super-resolution imaging of PcG bodies

Protected: 3D optics in thicker sections

Posted on February 27, 2012 by admin

There is no excerpt because this is a protected post.

Posted in Microscopy | Tagged , | Comments Off on Protected: 3D optics in thicker sections

Sunday 02-26-2012

Posted on February 26, 2012 by admin

9:00 A — 11:10 P Etching 4H sample. Testing 4H sample on STORM. 4H has very weak switching.  Might be new Glox not working.  Or extra resin?  see STORM post. Confocal of new in situs.  en-Pc H3-750 failed.  en still … Continue reading →

Posted in Summaries | Tagged | Comments Off on Sunday 02-26-2012

STORM 02-26-12

Posted on February 26, 2012 by admin

Laser Powers: 750: 100 mW 647: 15o mW 561: 150 mW 488: 15o mW sample ‘4H’ (histone H3 stained in all 4 channels as an alignment control). etched sample 22 min.  Looks like all resin is dissolved. very weak switching. … Continue reading →

Posted in Microscopy | Tagged | Comments Off on STORM 02-26-12

Friday 02/24/12

Posted on February 24, 2012 by admin

11:00A – 2:20A humidifying sectioning area.  Trimming 4H sample (all for H3 antibodies). 2 hr hot wash of embryos kept strict, followed by PBT rinse, long block (2 hrs+), then primaries 1 hr at RT, then O/N at 4C. taking … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Friday 02/24/12

STORM 2-24-12

Posted on February 24, 2012 by admin

Laser powers 750: fix to 100 mW 647: 175 mW 561 (at 200 mW activation) fix to 150 mW 488:  200 mW 405: 1.5 mW Imaged S6 500 nm, single section with hox expression dot. Arraying sample of S7, engrailed … Continue reading →

Posted in Microscopy | Tagged | Comments Off on STORM 2-24-12

STORM 02/21/12

Posted on February 22, 2012 by admin

  Laser powers 750: 102 mW 647: 200 mW 561: 150 mW 488: 200 mW 405: 1.5 mW imaging slide S7 750 looks pretty good, Dm01 better in young embryos. Other channels look like resin is still strong in outline. … Continue reading →

Posted in Microscopy | Tagged | Comments Off on STORM 02/21/12

STORM session: (hox slide 1)

Posted on February 16, 2012 by admin

STORM imaging 2/16/12 Laser calibration at output: 750 100 mW, 647 200 mW 561 100 mW (200 at laser) 488 200 mW 405 1.3 mW (max why low?) substantial resin still on slide S7 etched yesterday for 30 min. etched … Continue reading →

Posted in Microscopy | Tagged | Comments Off on STORM session: (hox slide 1)

Wednesday 02/15/12

Posted on February 15, 2012 by admin

9:45 A — 11:00P Etching Dm0-1 slides (25-30 min) for STORM.  (Slide 69 in records) Flip fly cage, 10A Washing out primary antibody in PcG stained embryos in situs for PcG stained embryos: hox and en locus (see records) organized … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Wednesday 02/15/12